GRP78 anticorps (Biotin)

N° du produit ABIN371046

Cet anticorps anti-GRP78 est un anticorps Rat Monoclonal détectant GRP78 dans WB, EIA et Func. Adapté pour Souris.

Aperçu rapide pour GRP78 anticorps (Biotin) (ABIN371046)

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP78 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Clone: 76-E6

Détail du produit anti-GRP78 anticorps (Biotin)

Specificité: This antibody is specific to BIP (Immunoglobulin heavy chain binding protein, Mr 78 kDa). Monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP. (Ref.8)

Attributs du produit: Synonyms: 78 kDa glucose-regulated protein, Heat shock 70 kDa protein 5, BiP, Immunoglobulinheavy chain-binding protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78

Isotype: IgG1

Information d'application

Indications d'application: ELISA. (Ref.6)Immunoblotting: < / = 10 μg/10^6 cells. (Ref.6,8)Studies of Immunoglobulin assembly and secretion. Ref.1,2)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur GRP78

Antigène: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Autre désignation: HSPA5 / GRP78

Sujet: The immunoglobulin heavy chain binding protein BiP (Binding Protein) is a member of the hsp70 family of heat shock proteins, and is identical to the glucose regulated protein grp78. (Ref.2) While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp 70 proteins have an essential physiological role in stress responses and as Molecular Chaperones which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. (Ref.1-5) With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (μ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. (Ref.7)Synonyms: 78 kDa glucose-regulated protein, BiP, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein

ID gène: 14828

UniProt: P20029

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling