LSD1 anticorps (AA 100-150)

N° du produit ABIN372401

L’anticorps Lapin Polyclonal anti-LSD1 a été validé pour WB et IHC (p). Il convient pour détecter LSD1 dans des échantillons de Humain, Souris, Rat, Singe, Chien, Macaque et Poisson zèbre (Danio rerio).

Aperçu rapide pour LSD1 anticorps (AA 100-150) (ABIN372401)

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivité: Humain, Souris, Rat, Singe, Chien, Macaque, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LSD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LSD1 anticorps

Épitope: AA 100-150

Specificité: This antibody recognizes Amine Oxidase (Flavin Containing) Domain 2 (AOF2/KDM1A).

Réactivité croisée (Details): Species reactivity (expected):Dog (Canine), Macaque, Monkey, Mouse, Rat and Zebrafish.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to a portion of amino acids of human AOF2.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 0.5-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur LSD1

Antigène: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Autre désignation: AOF2

Sujet: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.Synonyms: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1

Poids moléculaire: 93 kDa.

ID gène: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, L'effet Warburg