AQP2 anticorps (pSer261)

N° du produit ABIN372590

Il existe 2+ publications pour ce produit. L’anticorps anti-AQP2 Polyclonal Lapin est utilisé pour la détection de AQP2 dans des échantillons de Humain, Rat, Souris, Chien, Boeuf (Vache), Poulet et Non-Human Primate. Il a été validé pour WB et IHC (fro).

Aperçu rapide pour AQP2 anticorps (pSer261) (ABIN372590)

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Reactivité: Humain, Rat, Souris, Chien, Boeuf (Vache), Poulet, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-AQP2 anticorps

Épitope: pSer261

Specificité: Specific for ~29k AQP2 protein phosphorylated at Ser261. This antibody Also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Réactivité croisée (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of Rat Aquaporin 2.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-AQP2 (ABIN372590)

Bogum, Faust, Zühlke, Eichhorst, Moutty, Furkert, Eldahshan, Neuenschwander, von Kries, Wiesner, Trimpert, Deen, Valenti, Rosenthal, Klussmann: "Small-molecule screening identifies modulators of aquaporin-2 trafficking." dans: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 5, pp. 744-58, (2013) (PubMed).

Hoffert, Fenton, Moeller, Simons, Tchapyjnikov, McDill, Yu, Pisitkun, Chen, Knepper: "Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2." dans: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24617-27, (2008) (PubMed).

Détails sur AQP2

Antigène: AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))

Autre désignation: Aquaporin-2

Sujet: Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct

ID gène: 25386

NCBI Accession: NP_037041

UniProt: P34080

Pathways: Response to Water Deprivation