Dynamin 1 anticorps (pSer774)

N° du produit ABIN372616

Cet anticorps Mouton Polyclonal détecte spécifiquement Dynamin 1 dans WB, IF et IHC (fro). Il présente une réactivité envers Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet et Non-Human Primate.

Aperçu rapide pour Dynamin 1 anticorps (pSer774) (ABIN372616)

Antigène: Dynamin 1 (DNM1)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Non-Human Primate

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Dynamin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Dynamin 1 anticorps

Épitope: pSer774

Specificité: Specific for the ~95k Dynamin protein phosphorylated at Ser774. This antiboy labels the purified protein phosphorylated in vitro by CDK5 but not by PKC. Does not cross react with other purified substrates of CDK5 (e.g. Amphiphysin and Synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.

Réactivité croisée (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat Dynamin.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/1000. Immunohistochemistry (Fixed Cultured Neurons): 1/1000. (See Ref.3)Immunofluorescence (Fixed Cultured Neurons): 1/1000. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur Dynamin 1

Antigène: Dynamin 1 (DNM1)

Autre désignation: Dynamin-1

Sujet: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates Dynamin at Ser774 and Ser778 that are the phosphorylation sites on Dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on Dynamin is thought to play a key role in synaptic vesicle trafficking.Synonyms: DNM, DNM1

ID gène: 140694

NCBI Accession: NP_542420

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling