GRIN2B anticorps (pTyr1336)

N° du produit ABIN372686

Il existe 1 publication pour ce produit. L’anticorps anti-GRIN2B Polyclonal Lapin est utilisé pour la détection de GRIN2B dans des échantillons de Humain, Rat, Souris et Non-Human Primate. Il a été validé pour WB, IF et IHC (fro).

Aperçu rapide pour GRIN2B anticorps (pTyr1336) (ABIN372686)

Antigène: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivité: Humain, Rat, Souris, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRIN2B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GRIN2B anticorps

Épitope: pTyr1336

Specificité: This antibody is specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by lambda-phosphatase treatment.

Réactivité croisée (Details): Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-GRIN2B (ABIN372686)

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Détails sur GRIN2B

Antigène: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Autre désignation: NMDAR2B

Sujet: The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).Synonyms: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B

ID gène: 2904

NCBI Accession: NP_000825

UniProt: Q13224

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity