SYN1 anticorps (pSer62, pSer67)

N° du produit ABIN372719

Il existe 1 publication pour ce produit. L’anticorps anti-SYN1 Polyclonal Lapin est utilisé pour la détection de SYN1 dans des échantillons de Rat, Souris et Boeuf (Vache). Il a été validé pour WB et DB.

Aperçu rapide pour SYN1 anticorps (pSer62, pSer67) (ABIN372719)

Antigène: SYN1 (Synapsin I (SYN1))

Reactivité: Rat, Souris, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYN1 est non-conjugé

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-SYN1 anticorps

Épitope: pSer62, pSer67

Specificité: This antibody recognizes the ~78k Synapsin I doublet phosphorylated at Ser62,67. Immunolabeling of the Synapsin I band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.

Réactivité croisée (Details): Species reactivity (expected):Bovine (100 %) and Mouse (100 %).
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the Phospho Serine 62,67 of Synapsin I.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, None, 100 μg/mL BSA and 50 % Glycerol

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-SYN1 (ABIN372719)

Tallent, Varghis, Skorobogatko, Hernandez-Cuebas, Whelan, Vocadlo, Vosseller: "In vivo modulation of O-GlcNAc levels regulates hippocampal synaptic plasticity through interplay with phosphorylation." dans: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 174-81, (2008) (PubMed).

Détails sur SYN1

Antigène: SYN1 (Synapsin I (SYN1))

Autre désignation: Synapsin-1

Sujet: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987, Jovanovic et al., 1996).Synonyms: Brain protein 4.1, SYN1, Synapsin I

ID gène: 6853

NCBI Accession: NP_008881

UniProt: P17600