ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 130 kDa band seen in HeLa, Jurkat and NSO (mouse)cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Leung-Pineda, Huh, Piwnica-Worms: "DDB1 targets Chk1 to the Cul4 E3 ligase complex in normal cycling cells and in cells experiencing replication stress." dans: Cancer research, Vol. 69, Issue 6, pp. 2630-7, (2009) (PubMed).
Antigène
DDB1
(Damage Specific DNA Binding Protein 1 (DDB1))
anticorps DDBA, anticorps UV-DDB1, anticorps XAP1, anticorps XPCE, anticorps XPE, anticorps XPE-BF, anticorps DDB1, anticorps xpe, anticorps ddba, anticorps xap1, anticorps xpce, anticorps xpe-bf, anticorps uv-ddb1, anticorps ddb1, anticorps 127kDa, anticorps AA408517, anticorps p127-Ddb1, anticorps damage specific DNA binding protein 1, anticorps damage-specific DNA binding protein 1, 127kDa, anticorps DNA damage-binding protein 1, anticorps damage-specific DNA binding protein 1, anticorps DDB1, anticorps ddb1, anticorps LOC100187356, anticorps Ddb1, anticorps ddb-1
Sujet
Damage specific DNA binding protein (DDB) functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, purified human DDB protein was injected into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.Synonyms: DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, Damage-specific DNA-binding protein 1, HBV X-associated protein 1, UV-DDB 1, UV-damaged DNA-binding factor, XAP-1, XPCe, XPE-BF, Xeroderma pigmentosum group E-complementing protein