ALDH1A1 anticorps (C-Term)

N° du produit ABIN374078

Il existe 1 publication pour ce produit. L’anticorps anti-ALDH1A1 Polyclonal Chèvre est utilisé pour la détection de ALDH1A1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour ALDH1A1 anticorps (C-Term) (ABIN374078)

Antigène: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ALDH1A1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-ALDH1A1 anticorps

Épitope: C-Term

Séquence: C-EVKTVTVKIS QKNS

Specificité: This antibody detects ALDH1 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog) and Cow.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C-Terminus of the protein according to NP_000680.2. Genename: ALDH1A1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in human liver lysates(calculated MW of 54.5 kDa according to NP_000680.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-ALDH1A1 (ABIN374078)

Amann, Hofmann, Freudenberger, Holland-Cunz, Eichmüller, Bazhin: "Expression and activity of alcohol and aldehyde dehydrogenases in melanoma cells and in melanocytes." dans: Journal of cellular biochemistry, Vol. 113, Issue 3, pp. 792-9, (2012) (PubMed).

Détails sur ALDH1A1

Antigène: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Autre désignation: ALDH1

Sujet: ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum, this is the most vulnerable site in PD (Chung et al, 2005). Some studies also identify ALDH1A1 as a marker of cancer stem cells. Studies of breast cancer tissues showed that those that expressed a form of ALDH1A1 had the worst outcomes.Synonyms: ALDC, ALDH-E1, ALDH1A1, ALHDII, Aldehyde dehydrogenase cytosolic, Aldehyde dehydrogenase family 1 member A1, PUMB1, RALDH1, Retinal dehydrogenase 1

ID gène: 216

NCBI Accession: NP_000680

UniProt: P00352

Pathways: Dopaminergic Neurogenesis