RACGAP1 anticorps (C-Term)

N° du produit ABIN374167

Cet anticorps Chèvre Polyclonal détecte spécifiquement RACGAP1 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Boeuf (Vache) et Chien.

Aperçu rapide pour RACGAP1 anticorps (C-Term) (ABIN374167)

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivité: Humain, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RACGAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-RACGAP1 anticorps

Épitope: C-Term

Séquence: C-GRQGNFFASP MLK

Specificité: This antibody detects RACGAP1 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1. Genename: RACGAP1

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Human Testis lysates(calculated MW of 71.0 kDa according to NP_037409.2). The observed molecular weightcorresponds to earlier findings in literature with different antibodies (Toure et al. , J BiolChem 1998 Mar 13,273(11): 6019-23, PMID: 9497316). Immunohistochemistry: 1 μg/mL. In paraffin embedded Human Testis, shows nuclearstaining of many cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur RACGAP1

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Autre désignation: RACGAP1

Sujet: Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO, RAC, and CDC42. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.Synonyms: KIAA1478, MGCRACGAP, Male germ cell RacGap, Rac GTPase-activating protein 1

Poids moléculaire: 71kDa (NP_037409).

ID gène: 29127

NCBI Accession: NP_037409

UniProt: Q9H0H5

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor