APEX1 anticorps (N-Term)

N° du produit ABIN374178

Cet anticorps anti-APEX1 Polyclonal Chèvre (ABIN374178) détecte spécifiquement APEX1 dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain, Boeuf (Vache), Porc et Chien.

Aperçu rapide pour APEX1 anticorps (N-Term) (ABIN374178)

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivité: Humain, Boeuf (Vache), Porc, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APEX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-APEX1 anticorps

Épitope: N-Term

Séquence: PKRGKKGAVA EDGD-C

Specificité: This antibody detects APEX1 / REF-1 at N-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogène: Synthetic peptide from the N-Terminus of Human APEX1 / APE1 (NP_001632.2). Genename: APEX1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 35 kDa band observed in Human epidermoid fibroblastA431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.5 kDaaccording to NP_001632.2, NP_542379.1, NP_542380.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur APEX1

Antigène: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Autre désignation: APEX1 / REF-1

Sujet: Apurinic / apyrimidinic endonuclease (APE1 or REF1) is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. Alterations in APE1 levels have been described in some cancers. APE1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.Synonyms: APE, APEN, APEX, APEX nuclease, APX, Apurinic-apyrimidinic endonuclease 1, HAP1, Protein REF-1, REF1

ID gène: 328

NCBI Accession: NP_001632

UniProt: P27695

Pathways: Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus