Iba1 anticorps (C-Term, Isoform 1)

N° du produit ABIN374206

Cet anticorps Chèvre Polyclonal détecte spécifiquement Iba1 dans WB, IHC (p) et EIA. Il présente une réactivité envers Humain, Rat, Souris et Porc.

Aperçu rapide pour Iba1 anticorps (C-Term, Isoform 1) (ABIN374206)

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivité: Humain, Rat, Souris, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Iba1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Iba1 anticorps

Épitope: C-Term, Isoform 1

Specificité: This antibody is expected to recognizes Isoform 1 (NP_116573.1) and Isoform 3 (NP_001614.3) of AIF1.

Réactivité croisée (Details): Species reactivity (expected):Human, Porcine.
Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3. Genename: AIF1

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 16 kDa band observed in Rat Brain lysates (calculatedMW of 16.6 kDa according to Human NP_001614.3 and of 16.6 kDa according to RatNP_058892.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Iba1

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: AIF1

Sujet: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

ID gène: 199

NCBI Accession: NP_116573

UniProt: P55008

Pathways: Smooth Muscle Cell Migration