RAB11A anticorps (C-Term)

N° du produit ABIN374252

L’anticorps anti-RAB11A Polyclonal Chèvre est utilisé pour la détection de RAB11A dans des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été validé pour WB.

Aperçu rapide pour RAB11A anticorps (C-Term) (ABIN374252)

Antigène: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RAB11A est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-RAB11A anticorps

Épitope: C-Term

Séquence: TENKPKVQCC QNI

Specificité: This antibody detetcs RAB11A at C-term.

Réactivité croisée (Details): Species reactivity (expected):Human, Rat, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_004654.1 Genename: RAB11A

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Mouse Heart lysates (CalculatedMW of 24.6 kDa according to NP_004654.1). In transfected HEK293 transiently expressingRAB11A a band of approx.26 kDa is observed. This band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur RAB11A

Antigène: RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))

Autre désignation: RAB11A / RAB11

Sujet: Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.Synonyms: RAB-11, Ras-related protein Rab-11A, YL8

ID gène: 8766

NCBI Accession: NP_004654

UniProt: P62491

Pathways: Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity