BAZ2B anticorps (C-Term)
Aperçu rapide pour BAZ2B anticorps (C-Term) (ABIN374295)
Antigène
Voir toutes BAZ2B AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Séquence
- C-YFEKKWTDTF KVS
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Specificité
- This antibody detects BAZ2B at C-term.
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Réactivité croisée (Details)
- Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
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Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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Immunogène
- Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B
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Indications d'application
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Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))
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Autre désignation
- BAZ2B
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Sujet
- BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4
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ID gène
- 29994
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NCBI Accession
- NP_038478
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UniProt
- Q9UIF8
Antigène
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