TRIM5 anticorps (N-Term)

N° du produit ABIN374320

Cet anticorps Chèvre Polyclonal détecte spécifiquement TRIM5 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour TRIM5 anticorps (N-Term) (ABIN374320)

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TRIM5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRIM5 anticorps

Épitope: N-Term

Séquence: ASGILVNVKE EVTC

Specificité: This antibody detects TRIM5 / RNF88 at N-term. It is expected to recognise all three human isoforms of this protein.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1. Genename: TRIM5

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 37 kDa band observed in Human Brain lysates (calculatedMW of 37.7 kDa according to NP_149084.1, isoform delta). An additional faint 47 kDa bandconsistent with calculated MW of 40.7 kDa according to NP_149083.1 (isoform gamma) isalso observed. Immunohistochemistry: 1.0 μg/mL. In paraffin embedded Human Tonsil,stains a group of cells in the interfollicular area, below the epithelium of tonsillar criptaeand weak in germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur TRIM5

Antigène: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Autre désignation: TRIM5 / RNF88

Sujet: Synonyms: RING finger protein 88, Tripartite motif-containing protein 5

ID gène: 85363

NCBI Accession: NP_149023

UniProt: Q9C035

Pathways: Activation of Innate immune Response