FBXO43 anticorps (C-Term)

N° du produit ABIN374351

L’anticorps Chèvre Polyclonal anti-FBXO43 a été validé pour WB, IF, EIA et FACS. Il convient pour détecter FBXO43 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour FBXO43 anticorps (C-Term) (ABIN374351)

Antigène: FBXO43 (F-Box Protein 43 (FBXO43))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FBXO43 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS)

Détail du produit anti-FBXO43 anticorps

Épitope: C-Term

Séquence: C-PGSAQSKRNL KRL

Specificité: Recognizes EMI2.

Réactivité croisée (Details): Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FBXO43

Antigène: FBXO43 (F-Box Protein 43 (FBXO43))

Autre désignation: FBXO43

Sujet: Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B

ID gène: 286151

NCBI Accession: NP_001025031

UniProt: Q4G163