GRAF anticorps (C-Term)

N° du produit ABIN374371

Cet anticorps Chèvre Polyclonal détecte spécifiquement GRAF dans WB. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour GRAF anticorps (C-Term) (ABIN374371)

Antigène: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GRAF est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-GRAF anticorps

Épitope: C-Term

Séquence: C-KTGLIPENYV EFL

Specificité: This antibody detects ARHGAP26/OPHN1L at C-term. It is expected to recognise both reported isoforms.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Bovine (Cow).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_055886.1, NP_001129080.1. Genename: ARHGAP26

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 92.0 kDa according to NP_055886.1). An additional strong band ofunknown identity was also consistently observed at 10 kDa. This band was successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur GRAF

Antigène: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Autre désignation: ARHGAP26 / OPHN1L

Sujet: Synonyms: GRAF, GTPase regulator associated with focal adhesion kinase, KIAA0621, Oligophrenin-1-like protein, Rho GTPase-activating protein 26, Rho-type GTPase-activating protein 26

ID gène: 23092

NCBI Accession: NP_055886

UniProt: Q9UNA1