POLL anticorps (C-Term)

N° du produit ABIN374383

Cet anticorps Chèvre Polyclonal détecte spécifiquement POLL dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour POLL anticorps (C-Term) (ABIN374383)

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POLL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POLL anticorps

Épitope: C-Term

Séquence: C-LGLPYREPAE RDW

Specificité: This antibody detects DNA Polymerase Lambda at C-term.

Réactivité croisée (Details): Species reactivity (expected):MouseSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_037406.1 Genename: POLL

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave a band at approx 40 kDa in Human Liver andHepG2 extracts at 1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Small Intestine showsnuclear staining of enterocytes and of most cells in the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur POLL

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Autre désignation: DNA Polymerase lambda

Sujet: DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.Synonyms: DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda

ID gène: 27343

NCBI Accession: NP_037406

UniProt: Q9UGP5

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response