POLL anticorps (C-Term)
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- Antigène Voir toutes POLL Anticorps
- POLL (Polymerase (DNA Directed), lambda (POLL))
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Épitope
- C-Term
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Reactivité
- Humain, Souris
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp POLL est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Séquence
- C-LGLPYREPAE RDW
- Specificité
- This antibody detects DNA Polymerase Lambda at C-term.
- Réactivité croisée (Details)
- Species reactivity (expected):MouseSpecies reactivity (tested):Human
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogène
- Peptide from C Terminus of the protein sequence according to NP_037406.1 Genename: POLL
- Top Product
- Discover our top product POLL Anticorps primaire
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- Indications d'application
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Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave a band at approx 40 kDa in Human Liver andHepG2 extracts at 1 μg/mL. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Small Intestine showsnuclear staining of enterocytes and of most cells in the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Antigène
- POLL (Polymerase (DNA Directed), lambda (POLL))
- Autre désignation
- DNA Polymerase lambda (POLL Produits)
- Synonymes
- anticorps T10O24.13, anticorps T10O24_13, anticorps zgc:66320, anticorps zgc:77901, anticorps wu:fe49a04, anticorps beta-n, anticorps pol-kappa, anticorps BETAN, anticorps POLKAPPA, anticorps 1110003P06Rik, anticorps AV007317, anticorps DNA polymerase lambda (POLL), anticorps DNA polymerase lambda, anticorps polymerase (DNA directed), lambda, anticorps polymerase (DNA) lambda, anticorps Pol{lambda}, anticorps PTRG_00468, anticorps poll, anticorps POLL, anticorps MCYG_03796, anticorps LOC9325878, anticorps MGYG_02138, anticorps Poll
- Sujet
- DNA Polymerase lambda is a family X member repair polymerase with low frameshift fidelity that is involved in base excision repair (BER) and is responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Polymerase lambda is the primary gap filling polymerase for accurate nonhomologous end joining, and the Brca1 C terminal domain is required for this activity.Synonyms: DNA polymerase beta-2, DNA polymerase kappa, POLL, Pol Lambda
- ID gène
- 27343
- NCBI Accession
- NP_037406
- UniProt
- Q9UGP5
- Pathways
- Réparation de l'ADN, Production of Molecular Mediator of Immune Response
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