ACTR1B anticorps (C-Term)

N° du produit ABIN374429

L’anticorps anti-ACTR1B Polyclonal produit chez le Chèvre a été validé pour WB et IHC (p). Il convient pour la détection de ACTR1B dans des échantillons provenant de Humain, Souris, Rat, Chien et Boeuf (Vache). }

Aperçu rapide pour ACTR1B anticorps (C-Term) (ABIN374429)

Antigène: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ACTR1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ACTR1B anticorps

Épitope: C-Term

Séquence: C-YEEDGSRAIH RKT

Specificité: This antibody recognizes ARP1 homolog B.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_005726. Genename: ACTR1B

Information d'application

Indications d'application: Peptide ELISA: 1/128000. Western Blot: 0.5-2 μg/mL. Approx 45 kDa band observed in human heart and brain lysates(predicted MW of 45 kDa according to NP_005726). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrialcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ACTR1B

Antigène: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Autre désignation: Centractin beta

Sujet: In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90 % amino acid identity and 96 % amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be involved in dynein-driven vesicle movement.Synonyms: ACTR1B, ARP1B, Actin-related protein 1B, CTRN2

ID gène: 10120

NCBI Accession: NP_005726

UniProt: P42025