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GAPDH anticorps (C-Term)

GAPDH Reactivité: Humain, Souris, Rat, Chien WB, IF, IHC (p), EIA Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN374435
  • Antigène Voir toutes GAPDH Anticorps
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Épitope
    • 37
    • 21
    • 13
    • 9
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 223
    • 142
    • 135
    • 75
    • 65
    • 55
    • 36
    • 28
    • 23
    • 21
    • 20
    • 19
    • 18
    • 16
    • 12
    • 12
    • 11
    • 10
    • 9
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Chien
    Hôte
    • 170
    • 97
    • 17
    • 10
    • 4
    Chèvre
    Clonalité
    • 191
    • 105
    Polyclonal
    Conjugué
    • 157
    • 36
    • 23
    • 17
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GAPDH est non-conjugé
    Application
    • 244
    • 98
    • 84
    • 70
    • 46
    • 41
    • 41
    • 35
    • 16
    • 16
    • 15
    • 15
    • 8
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    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Séquence
    C-HQVVSSDFNS DT
    Specificité
    This antibody is specific to GAPDH at C-Term. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a Loading/Positive Control is required in Western blotting.
    Réactivité croisée (Details)
    Species reactivity (expected):Canine (Dog).
    Species reactivity (tested):Human, Mouse, Rat.
    Purification
    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
    Immunogène
    Peptide with sequence from the C-Terminus of the protein sequence according to NP_002037.2.
    Top Product
    Discover our top product GAPDH Anticorps primaire
  • Indications d'application
    Peptide ELISA: Detection Limit: 1/2000Western Blot: 0.01-0.03 μg/mL. A approx. 35 kDa band is observed in lysates of Human Tonsil,MouseSpleen, and cell lines HeLa, A431 and 293. Immunohistochemistry In Paraffin Embedded Tonsil: 0.3 μg/mL. Staining of cells ingerminal centre, mantle zone, interfollicular area, epithelium and endothelium is
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Farris, Pifer, Zheng, Gottlieb, Denvir, Frisch: "Grainyhead-like 2 Reverses the Metabolic Changes Induced by the Oncogenic Epithelial-Mesenchymal Transition: Effects on Anoikis." dans: Molecular cancer research : MCR, Vol. 14, Issue 6, pp. 528-38, (2017) (PubMed).

    Breitenbach, Gruber, Klausegger, Trost, Bogner, Reitsamer, Bauer: "Pseudosyndactyly - an inflammatory and fibrotic wound healing disorder in recessive dystrophic epidermolysis bullosa." dans: Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG, Vol. 13, Issue 12, pp. 1257-66, (2016) (PubMed).

    Herzog, Rid, Wagner, Hundsberger, Eger, Bauer, Önder: "Whole-transcriptome gene expression profiling in an epidermolysis bullosa simplex Dowling-Meara model keratinocyte cell line uncovered novel, potential therapeutic targets and affected pathways." dans: BMC research notes, Vol. 8, pp. 785, (2016) (PubMed).

    Happold, Roth, Silginer, Florea, Lamszus, Frei, Deenen, Reifenberger, Weller: "Interferon-? induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells." dans: Molecular cancer therapeutics, Vol. 13, Issue 4, pp. 948-61, (2014) (PubMed).

    Xu, Weerachayaphorn, Cai, Soroka, Boyer: "Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 299, Issue 1, pp. G126-35, (2010) (PubMed).

    Kiepe, Van Der Pas, Ciarmatori, Ständker, Schütt, Hoeflich, Hügel, Oh, Tönshoff: "Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein-2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I." dans: Endocrinology, Vol. 149, Issue 10, pp. 4901-11, (2008) (PubMed).

    Gerstner, Bremer, Vander Heyden, Lavaute, Yin, Landry: "Brain fatty acid binding protein (Fabp7) is diurnally regulated in astrocytes and hippocampal granule cell precursors in adult rodent brain." dans: PLoS ONE, Vol. 3, Issue 2, pp. e1631, (2008) (PubMed).

    Henson, Roberts, Salimi, Vadlamudi, Hamer, Gilmore, Jarskog, Philpot: "Developmental regulation of the NMDA receptor subunits, NR3A and NR1, in human prefrontal cortex." dans: Cerebral cortex (New York, N.Y. : 1991), Vol. 18, Issue 11, pp. 2560-73, (2008) (PubMed).

    Nguyen, Pace, Koller: "Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia." dans: The Journal of experimental medicine, Vol. 204, Issue 6, pp. 1383-93, (2007) (PubMed).

    Hagiwara, Tajika, Matsuzaki, Suzuki, Aoki, Takata: "Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome." dans: Histochemistry and cell biology, Vol. 126, Issue 2, pp. 251-9, (2006) (PubMed).

  • Antigène
    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
    Autre désignation
    GAPDH (GAPDH Produits)
    Synonymes
    anticorps G3PD, anticorps GAPD, anticorps Gapd, anticorps BEST:GH12586, anticorps CG12055, anticorps Dmel\\CG12055, anticorps GA3PDH, anticorps GADPH, anticorps GAP, anticorps GAPDH, anticorps GAPDH I, anticorps GAPDH-1, anticorps GAPDH1, anticorps GAPDHI, anticorps Gapdh, anticorps Gapdh-1, anticorps Gapdh43E, anticorps gadph, anticorps gapdh, anticorps gapdh-1, anticorps gh12586, anticorps cb609, anticorps gapd, anticorps mg:bb02e05, anticorps wu:fb33a10, anticorps wu:ft80f05, anticorps KNC-NDS6, anticorps g3pd, anticorps G3PDH, anticorps glyceraldehyde-3-phosphate dehydrogenase, anticorps Glyceraldehyde 3 phosphate dehydrogenase 1, anticorps glyceraldehyde-3-phosphate dehydrogenase S homeolog, anticorps glyceraldehyde-3-phosphate dehydrogenase, type I, anticorps olfactory receptor 8K3, anticorps GAPDH, anticorps Gapdh, anticorps Gapdh1, anticorps gapdh, anticorps gapdh.S, anticorps gapDH, anticorps LOC100404960, anticorps LOC614985
    Sujet
    Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a metabolic enzyme originally thought to be only involved in Glycolysis. GAPDH is constitutively expressed at high levels in almost all tissues and serves therefore as a useful marker for the measurement of equal loading in western blotting experiments. Because of its ubiquitous expression, GAPDH is often seen as a housekeeping gene. However in the last two decades, every year a new function for this enzyme has been revealed. Besides its central role as a cytoplasmic enzyme in Glycolysis, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as DNA replication and repair, nuclear membrane fusion, endocytosis, nuclear translocation in apoptosis, microtubule bundling, vesicular secretory transport, maintenance of telomere structure, phosphotransferase activity and nuclear RNA export. We now know that GAPDH is not only in the cytoplasm, but it shuttles between the cytoplasm and the nucleus and membranes. A lot of reports demonstrate the role of GAPDH in different pathologies such as progression in prostate cancer and ovarian cancer (mRNA stability), programmed neuronal cell death, and agerelated neuronal diseases. For example the sub-cellular distribution of GAPDH is abnormal in patients with Alzheimer's disease.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
    Poids moléculaire
    36.1 kDa (Calculated)
    ID gène
    2597
    NCBI Accession
    NP_002037
    UniProt
    P04406
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