BIM anticorps (C-Term)

N° du produit ABIN374444

L’anticorps anti-BIM Polyclonal Chèvre est utilisé pour la détection de BIM dans des échantillons de Humain. Il a été validé pour WB.

Aperçu rapide pour BIM anticorps (C-Term) (ABIN374444)

Antigène: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BIM est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-BIM anticorps

Épitope: C-Term

Séquence: C-FNAYYARRLE K

Specificité: This antibody is expected to recognise 3 reported isoforms: BimAD, BimACD and BimABCD.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_619528, NP_996886, NP_619529.

Information d'application

Indications d'application: Peptide ELISA: 1/16,000. Western Blot: 0.5-2 μg/mL. Detects a band of Approx 23 kDa in K562 lysates (PredictedMolecular Weight: 19.9, 12 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur BIM

Antigène: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Sujet: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

ID gène: 10018, 9606

UniProt: O43521

Pathways: Signalisation PI3K-Akt, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity