BID anticorps (C-Term)

N° du produit ABIN374543

L’anticorps anti-BID Polyclonal Chèvre est utilisé pour la détection de BID dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour BID anticorps (C-Term) (ABIN374543)

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BID est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-BID anticorps

Épitope: C-Term

Séquence: C-TYVRSLARNG MD

Specificité: This antibody reacts to BH3-interacting domain death agonist. It is expected to recognise all three reported isoforms (as represented by NP_932070.1, NP_001187.1, NP_932071.1)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: Peptide with sequence from the C Terminus of the protein sequence according to NP_932070.1, NP_001187.1, NP_932071.1. Genename: BID

Information d'application

Indications d'application: Peptide ELISA: 1/8000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur BID

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Autre désignation: BID

Sujet: Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.Synonyms: BH3-interacting domain death agonist, p22 BID

Poids moléculaire: 26.8kDa (NP_932070.1) and 22.0kDa (NP_001187.1).

ID gène: 637, 9606

UniProt: P55957

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity