DARC anticorps (N-Term)

N° du produit ABIN374609

L’anticorps Chèvre Polyclonal anti-DARC a été validé pour WB, FACS et IHC (p). Il convient pour détecter DARC dans des échantillons de Humain.

Aperçu rapide pour DARC anticorps (N-Term) (ABIN374609)

Antigène: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DARC est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DARC anticorps

Épitope: N-Term

Specificité: This antibody recognizes Duffy/FY/DARC (N-term).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence HRAELSPSTENSSQLDFED-C, from the N Terminus of the protein sequence according to NP_002027.2. Genename: DARC

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 35 kDa band observed in human kidney and humanliver lysates (calculated MW of 35.5 kDa according to NP_002027.2). Immunohistochemistry: 3 μg/mL. In paraffin embedded human kidney, stains epithelialcells of renal tubules and endothelial cells of vessels. The granular staining is compatiblewith the endoplasmic reticulum association of the protein. This antibody has also beensuccessfully used on Huamn Lung showing endothelial staining. FACS: A customer has reported Successful staining of HEK293S-TetR transfected with DARCreceptor at a dilution of 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur DARC

Antigène: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Autre désignation: CD234 / DARC

Sujet: DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system.Synonyms: Duffy antigen/chemokine receptor, FY, Fy glycoprotein, GPD, Glycoprotein D, GpFy, Plasmodium vivax receptor

ID gène: 2532, 9606

UniProt: Q16570