IL12B anticorps (Internal Region)

N° du produit ABIN374765

L’anticorps anti-IL12B Polyclonal Chèvre est utilisé pour la détection de IL12B dans des échantillons de Humain. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour IL12B anticorps (Internal Region) (ABIN374765)

Antigène: IL12B (Interleukin 12 beta (IL12B))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL12B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-IL12B anticorps

Épitope: Internal Region

Séquence: C-QGKSKREKKD RVFTD

Specificité: This antibody recognizes Interleukin-12B (IL12B).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_002178.2.

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in Human Liver lysates(calculated MW of 37.7 kDa according to NP_002178.2). Immunohistochemistry on Paraffin Embedded Sections: 4-6 μg/mL. In paraffin embedded Human Colon shows cytoplasm staining of individual cells betweencolumns. In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of someDCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IL12B

Antigène: IL12B (Interleukin 12 beta (IL12B))

Autre désignation: Interleukin-12 beta / IL12B

Sujet: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

ID gène: 3593

NCBI Accession: NP_002178

UniProt: P29460

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation