FH anticorps (Internal Region)

N° du produit ABIN374767

Cet anticorps Chèvre Polyclonal détecte spécifiquement FH dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour FH anticorps (Internal Region) (ABIN374767)

Antigène: FH (Fumarate Hydratase (FH))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FH est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-FH anticorps

Épitope: Internal Region

Séquence: C-HPNDHVNKSQ SSND

Specificité: This antibody recognizes Fumarase/FH

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_000134.2 Genename: FH

Information d'application

Indications d'application: Peptide ELISA: 1/64000. Western Blot: 0.01-0.03 μg/mL. Approx 48 kDa band observed in Human Kidney, Liver andLung lysates. The observed molecular weight corresponds to earlier findings withantibodies from different sources. In transfected HEK293 transiently expressing Fumarasea band of approx.58 kDa is observed. This band is not observed in the non-transfectedHEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FH

Antigène: FH (Fumarate Hydratase (FH))

Autre désignation: Fumarase

Sujet: FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.Synonyms: HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase

Poids moléculaire: 54.6 kDa (NP_000134.2).

ID gène: 2271, 9606

UniProt: P07954