Glucose-6-Phosphate Dehydrogenase anticorps (Internal Region)
Aperçu rapide pour Glucose-6-Phosphate Dehydrogenase anticorps (Internal Region) (ABIN374790)
Antigène
Voir toutes Glucose-6-Phosphate Dehydrogenase (G6PD) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 308-320, Internal Region
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Séquence
- C-STNSDDVRDE KVK
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Specificité
- This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Rat and Dog (Canine).
Species reactivity (tested):Human. -
Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.
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Immunogène
- Synthetic Peptide from the internal region (aa 308-320) of Human G6PD. Genename: G6PD
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Indications d'application
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Peptide ELISA: Limit dilution: 1/32000. Western Blot: Use at 0.03-0.1 μg/mL. Detects a double band of approx. 55-60 kDa band in HumanTestis, Thyroid and Tonsil lysates. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Testis shows strongcytoplasm staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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Autre désignation
- G6PD
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Sujet
- Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase
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ID gène
- 2539, 9606
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UniProt
- P11413
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Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones
Antigène
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