PCK1 anticorps (C-Term, Internal Region)

N° du produit ABIN374797

Cet anticorps Chèvre Polyclonal détecte spécifiquement PCK1 dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour PCK1 anticorps (C-Term, Internal Region) (ABIN374797)

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PCK1 anticorps

Épitope: C-Term, Internal Region

Séquence: C-EKEVEDIEKY LEDQ

Specificité: This antibody detects PEP carboxykinase.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2. Genename: PCK1

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-1.5 μg/mL. Approx 70 kDa band observed in Human Kidney lysates(calculated MW of 69.0 kDa according to NP_002582.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1 / PEPCK1

Sujet: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.Synonyms: PEP carboxykinase, PEPCK-C, PEPCKC, Phosphoenolpyruvate carboxykinase cytosolic [GTP], Phosphoenolpyruvate carboxylase

ID gène: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis