ABHD5 anticorps (Internal Region)

N° du produit ABIN374842

Cet anticorps Chèvre Polyclonal détecte spécifiquement ABHD5 dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour ABHD5 anticorps (Internal Region) (ABIN374842)

Antigène: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ABHD5 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ABHD5 anticorps

Épitope: Internal Region

Specificité: This antibody recognizes CGI58 / ABHD5. AP16647PU-N is not expected to cross-react with ABHD4.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogène: Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence Genename: ABHD5

Information d'application

Indications d'application: Peptide ELISA: ABHD5 Antibody detection limit dilution: 1/16000. Western Blot: 0.2-0.6 μg/mL. Approx 40 kDa band observed in lysates of cell lines NIH3T3and A431 calculated MW of 39.0 kDa according to NP_057090.2). Additional faint bands of20 kDa and 10 kDa were observed. These bands were successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ABHD5

Antigène: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Autre désignation: ABHD5

Sujet: ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. ABHD5 is widely expressed in various tissues, including skin, lymphocytes, liver, skeletal muscle and brain.Synonyms: Abhydrolase domain-containing protein 5, CGI-58, NCIE2

ID gène: 51099, 9606

UniProt: Q8WTS1

Pathways: Lipid Metabolism