SMN1 / SMN2 (all Isoforms), (Internal Region) anticorps

N° du produit ABIN374850

Cet anticorps anti- est un anticorps Chèvre Polyclonal détectant dans WB. Adapté pour Humain.

Aperçu rapide pour SMN1 / SMN2 (all Isoforms), (Internal Region) anticorps (ABIN374850)

Antigène: SMN1 / SMN2

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB)

Détail du produit

Épitope: all Isoforms, Internal Region

Séquence: C-DESENSRSPG NKSDN

Specificité: This antibody is expected to recognise isoforms b and d of SMN1 (NP_075012.1 and NP_000335.1) and all reported isoforms of SMN2 (NP_075013.1, NP_075014.1, NP_075015.1 and NP_059107.1).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Synthetic peptide corresponding to internal region of Human SMN1+SMN2 according to NP_075012.1, NP_000335.1, NP_075013.1, NP_075014.1, NP_075015.1, NP_059107.1.

Information d'application

Indications d'application: Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.3-1 μg/mL. Approx 26 kDa band observed in Human Brain (Cerebellum)lysates (calculated MW of 28.6 kDa according to NP_075012.1 and NP_075014.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: SMN1 / SMN2

Sujet: The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly.Synonyms: Component of gems 1, Gemin-1, Gemin1, SMN, SMNC, SMNT, Survival motor neuron protein

ID gène: 6606

NCBI Accession: NP_000335

UniProt: Q16637