alpha Tubulin anticorps

N° du produit ABIN387714

Cet anticorps anti-alpha Tubulin est un anticorps Souris Monoclonal détectant alpha Tubulin dans WB et ELISA. Adapté pour Humain, Souris, Porc et Hamster. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour alpha Tubulin anticorps (ABIN387714)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Souris, Porc, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 1F4E3

Détail du produit anti-alpha Tubulin anticorps

Specificité: Anti-alpha-tubulin Monoclonal Antibody (Mouse) reacts with mouse, human, hamster, monkey, and pig alpha-tubulin. This product has not yet been tested in other species.

Réactivité croisée (Details): Cross reactivity in other species has not yet been tested.

Purification: Protein A affinity column

Immunogène: A synthetic peptide (ALEKDYEEVGVDSVE) coupled - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunofluorescent staining: 1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Références pour anticorps anti-alpha Tubulin (ABIN387714)

Amita, Adachi, Alexenko, Sinha, Schust, Schulz, Roberts, Ezashi: "Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 13, pp. E1212-21, (2013) (PubMed).

Chen, Cheng, Liu, King, Peng, Zhang, Wang, Shresta, Schooley, Liu: "Inhibitory and combinatorial effect of diphyllin, a v-ATPase blocker, on influenza viruses." dans: Antiviral research, Vol. 99, Issue 3, pp. 371-82, (2013) (PubMed).

Kuo, Wang, Lee, Iwasaki, Duque-Afonso, Wong, Lin, Figueroa, Su, Lemischka, Cleary: "Epigenetic roles of MLL oncoproteins are dependent on NF-?B." dans: Cancer cell, Vol. 24, Issue 4, pp. 423-37, (2013) (PubMed).

Lakshmana, Hayes, Bennett, Bianchi, Reddy, Koo, Kang: "Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 5, pp. 2072-83, (2012) (PubMed).

Schmid, Lehmann, Lucius, Gupta: "Apicomplexan parasite, Eimeria falciformis, co-opts host tryptophan catabolism for life cycle progression in mouse." dans: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20197-207, (2012) (PubMed).

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha Tubulin

Sujet: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Microtubules are obligate proteinaceous elements found in nearly all eukaryotic cells. The availability of monoclonal antibodies to alpha-tubulin uses as a common housekeeping protein and serves as a specific and useful tool in studying proper interpretation of western blots.

Pathways: Dynamique des Microtubules