DYKDDDDK Tag anticorps (FITC)

N° du produit ABIN387724

Cet anticorps Souris Monoclonal détecte spécifiquement DYKDDDDK Tag dans IF et FACS. Il présente une réactivité envers et a été mentionné dans 1 publication.

Aperçu rapide pour DYKDDDDK Tag anticorps (FITC) (ABIN387724)

Antigène: DYKDDDDK Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYKDDDDK Tag est conjugé à/à la FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5A8E5

Détail du produit anti-DYKDDDDK Tag anticorps (FITC)

Marque: THE™

Séquence: DYKDDDDK

Specificité: THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.

Attributs du produit: This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Purification: Protein A affinity column

Immunogène: A synthetic peptide DYKDDDDK coupled - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/mL
Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-DYKDDDDK Tag (FITC) (ABIN387724)

Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." dans: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, (2012) (PubMed).

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Classe de substances: Tag