Kdm6b anticorps (N-Term)

N° du produit ABIN387862

Cet anticorps Lapin Polyclonal détecte spécifiquement Kdm6b dans WB, IF et IHC (p). Il présente une réactivité envers Humain et a été mentionné dans 15+ publications.

Aperçu rapide pour Kdm6b anticorps (N-Term) (ABIN387862)

Antigène: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Kdm6b est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB10077

Détail du produit anti-Kdm6b anticorps

Épitope: AA 1-30, N-Term

Homologie: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Date de péremption: 6 months

Références pour anticorps anti-Kdm6b (ABIN387862)

Angrisano, Pero, Brancaccio, Coretti, Florio, Pezone, Calabrò, Falco, Keller, Lembo, Avvedimento, Chiariotti: "Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells." dans: PLoS ONE, Vol. 11, Issue 6, pp. e0156671, (2017) (PubMed).

Petruk, Mariani, De Dominici, Porazzi, Minieri, Cai, Iacovitti, Flomenberg, Calabretta, Mazo: "Structure of Nascent Chromatin Is Essential for Hematopoietic Lineage Specification." dans: Cell reports, Vol. 19, Issue 2, pp. 295-306, (2017) (PubMed).

Liao, Kuo, Lu, Wang, Wu: "Generation of an anti-EpCAM antibody and epigenetic regulation of EpCAM in colorectal cancer." dans: International journal of oncology, Vol. 46, Issue 4, pp. 1788-800, (2015) (PubMed).

Hashizume, Andor, Ihara, Lerner, Gan, Chen, Fang, Huang, Tom, Ngo, Solomon, Mueller, Paris, Zhang, Petritsch, Gupta, Waldman, James: "Pharmacologic inhibition of histone demethylation as a therapy for pediatric brainstem glioma." dans: Nature medicine, (2014) (PubMed).

Lee, Na, Lee, Na, Cho, Wu, Yune, Kim, Ju: "Molecular mechanism of Jmjd3-mediated interleukin-6 gene regulation in endothelial cells underlying spinal cord injury." dans: Journal of neurochemistry, Vol. 122, Issue 2, pp. 272-82, (2013) (PubMed).

Ramadoss, Chen, Wang: "Histone demethylase KDM6B promotes epithelial-mesenchymal transition." dans: The Journal of biological chemistry, Vol. 287, Issue 53, pp. 44508-17, (2012) (PubMed).

Balasubramanian, Chew, Eckert: "Sulforaphane suppresses polycomb group protein level via a proteasome-dependent mechanism in skin cancer cells." dans: Molecular pharmacology, Vol. 80, Issue 5, pp. 870-8, (2011) (PubMed).

Hyland, McDade, McCloskey, Dickson, Arthur, McCance, Patel: "Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in human papillomavirus type 16 E6/E7-expressing keratinocytes." dans: Journal of virology, Vol. 85, Issue 21, pp. 10999-1006, (2011) (PubMed).

Kim, Yoon, Chuong, Oyolu, Wills, Gupta, Baker: "Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs." dans: Developmental biology, Vol. 357, Issue 2, pp. 492-504, (2011) (PubMed).

Lu, Lu, Liao, Yu, Chung, Kao, Wu: "Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells." dans: The Journal of biological chemistry, Vol. 285, Issue 12, pp. 8719-32, (2010) (PubMed).

Dai, Lu, Zhang, Shen: "Jmjd3 activates Mash1 gene in RA-induced neuronal differentiation of P19 cells." dans: Journal of cellular biochemistry, Vol. 110, Issue 6, pp. 1457-63, (2010) (PubMed).

Toth, Maglinte, Lee, Lee, Wong, Brulois, Lee, Buckley, Laird, Marquez, Jung: "Epigenetic analysis of KSHV latent and lytic genomes." dans: PLoS pathogens, Vol. 6, Issue 7, pp. e1001013, (2010) (PubMed).

Peláez, Kalogeropoulou, Ferraro, Voulgari, Pankotai, Boros, Pintzas: "Oncogenic RAS alters the global and gene-specific histone modification pattern during epithelial-mesenchymal transition in colorectal carcinoma cells." dans: The international journal of biochemistry & cell biology, Vol. 42, Issue 6, pp. 911-20, (2010) (PubMed).

Long, Huang, Yin, Zhao, Zhao, Lu: "Abnormal expression pattern of histone demethylases in CD4(+) T cells of MRL/lpr lupus-like mice." dans: Lupus, Vol. 18, Issue 14, pp. 1327-8, (2009) (PubMed).

Ishii, Wen, Corsa, Liu, Coelho, Allen, Carson, Cavassani, Li, Lukacs, Hogaboam, Dou, Kunkel: "Epigenetic regulation of the alternatively activated macrophage phenotype." dans: Blood, Vol. 114, Issue 15, pp. 3244-54, (2009) (PubMed).

Détails sur Kdm6b

Antigène: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Autre désignation: JMJD3

Sujet: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Poids moléculaire: 176632

ID gène: 23135

NCBI Accession: NP_001073893

UniProt: O15054

Pathways: L'effet Warburg