HRH3 anticorps (C-Term)

N° du produit ABIN388438

Détail du produit anti-HRH3 anticorps

Antigène: HRH3 (Histamine Receptor H3 (HRH3))

Épitope: AA 414-442, C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HRH3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This HRH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human HRH3.

Clone: RB01071

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Détails sur HRH3

Antigène: HRH3 (Histamine Receptor H3 (HRH3))

Autre désignation: HRH3 (HRH3 Produits)

Synonymes: anticorps GPCR97, anticorps HH3R, anticorps AW049250, anticorps H3R, anticorps histamine receptor H3, anticorps HRH3, anticorps Hrh3

Sujet: The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. HRH3 has significant sequence homology to members of the biogenic amine subfamily of GPCRs. Most notable is an aspartic acid residue in the predicted third transmembrane domain, which is a hallmark of the biogenic amine receptor subfamily, this residue is the putative binding site for the primary amine. HRH3 shares 22 % and 21.4 % amino acid sequence homology with the H1 and H2 receptors, respectively. Expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Northern blot analysis of human tissues showed HRH3 expression only in the brain, with highest expression in the thalamus and caudate nucleus. Whereas Northern blot analysis did not detect HRH3 expression in any peripheral tissue examined, RT-PCR showed expression in human small intestine, testis, and prostate. In situ hybridization of rat brain sections showed that Hrh3 is abundantly expressed in brain. Hrh3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle. In addition, Hrh3 expression was found in the locus ceruleus and in the histaminergic cell bodies in the tuberomammillary nuclei.

Poids moléculaire: 48671

ID gène: 11255

NCBI Accession: NP_009163

UniProt: Q9Y5N1

Pathways: cAMP Metabolic Process, Feeding Behaviour