PPT1 anticorps (N-Term)

N° du produit ABIN389116

Cet anticorps Lapin Polyclonal détecte spécifiquement PPT1 dans WB. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour PPT1 anticorps (N-Term) (ABIN389116)

Antigène: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPT1 est non-conjugé

Application: Western Blotting (WB)

Clone: RB4706

Détail du produit anti-PPT1 anticorps

Épitope: AA 32-63, N-Term

Homologie: B, Pr

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-63 amino acids from the N-terminal region of human PPT1.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Détails sur PPT1

Antigène: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Autre désignation: PPT1

Sujet: Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18 % amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle, variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

Poids moléculaire: 34193

ID gène: 5538

NCBI Accession: NP_000301, NP_001136076

UniProt: P50897

Pathways: SARS-CoV-2 Protein Interactome