Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
Specificité
This Phospho-Rb-S788 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S788 of human Rb.
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This Rb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S788 of human Rb.
RB1
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IF, ICC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
DB = 1:500, IHC (p) = 1:50-100
Restrictions
For Research Use only
Format
Liquid
Concentration
0.45 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C/-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Iwahori, Hakki, Chou, Kalejta: "Molecular Determinants for the Inactivation of the Retinoblastoma Tumor Suppressor by the Viral Cyclin-dependent Kinase UL97." dans: The Journal of biological chemistry, Vol. 290, Issue 32, pp. 19666-80, (2015) (PubMed).
RB1 likely acts as a regulator of other genes. It forms a complex with adenovirus E1A and with SV40 large T antigen, acts as a tumor suppressor, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RB1 is a potent inhibitor of E2F-mediated trans-activation, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression. This protein inhibits the intrinsic kinase activity of TAF1. Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), a congenital malignant tumor that arises from the nuclear layers of the retina. Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma. Synonyms: Retinoblastoma-associated protein, PP110, P105-RB, RB, RB1,