Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogène
This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota.
PRKCI
Reactivité: Humain, Souris, Rat, Chien, Poulet
WB, IHC, IP, IF
Hôte: Souris
Monoclonal
41-PKClambda
unconjugated
Indications d'application
WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Lechuga, Baranwal, Ivanov: "Actin-interacting protein 1 controls assembly and permeability of intestinal epithelial apical junctions." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 308, Issue 9, pp. G745-56, (2015) (PubMed).
Wang, Li, Du, Lu, Zou: "Significance and expression of atypical protein kinase C-iota in human hepatocellular carcinoma." dans: The Journal of surgical research, Vol. 154, Issue 1, pp. 143-9, (2009) (PubMed).
Li, Wang, Liu, Xiao, Lu, Zou: "Correlation of aPKC-iota and E-cadherin expression with invasion and prognosis of cholangiocarcinoma." dans: Hepatobiliary & pancreatic diseases international : HBPD INT, Vol. 7, Issue 1, pp. 70-5, (2008) (PubMed).
PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.