P4HB anticorps (AA 499-509)

N° du produit ABIN396933

Cet anticorps anti-P4HB est un anticorps Souris Monoclonal détectant P4HB dans WB, IHC, ICC et IHC (p). Adapté pour Humain, Souris, Rat, Hamster, Boeuf (Vache), Chien, Singe, Cobaye, Porc, Mouton, Lapin, Xenopus laevis et Poulet.

Aperçu rapide pour P4HB anticorps (AA 499-509) (ABIN396933)

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivité: Humain, Souris, Rat, Hamster, Boeuf (Vache), Chien, Singe, Cobaye, Porc, Mouton, Lapin, Xenopus laevis, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp P4HB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-P4HB anticorps

Épitope: AA 499-509

Marque: IHC-plus™

Specificité: Recognizes rat protein disulfide isomerase at ~58kD.

Purification: Protein G purified

Immunogène: Synthetic peptide corresponding to aa499-509 of rat protein disulfide isomerase (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: ICC, IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate, MCF-7 cell lysate.

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur P4HB

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: PDI / P4HB

Sujet: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

ID gène: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism