APEX1 anticorps
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- Antigène Voir toutes APEX1 Anticorps
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp APEX1 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Marque
- IHC-plus™
- Specificité
- Specific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Inhibits repair activity of ape protein.
- Purification
- Immunoaffinity purified
- Immunogène
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Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.
Type of Immunogen: Fusion protein - Isotype
- IgG
- Top Product
- Discover our top product APEX1 Anticorps primaire
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- Indications d'application
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Approved: IHC, IHC-Fr, IHC-P (1:250), WB (1:500)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis. Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. Positive control: HeLa whole cell extract. - Commentaires
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 0.05 % sodium azide before the addition of glycerol to 40 %.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Antigène
- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
- Autre désignation
- APEX1 / APE1 (APEX1 Produits)
- Synonymes
- anticorps apex1, anticorps MGC76280, anticorps MGC79645, anticorps MGC78928, anticorps APE, anticorps APE1, anticorps APEN, anticorps APEX, anticorps APX, anticorps HAP1, anticorps REF1, anticorps Apex, anticorps REF-1, anticorps BAP1, anticorps apex1a, anticorps hm:zehn1532, anticorps wu:fc13d11, anticorps zgc:66204, anticorps Ref-1, anticorps apurinic/apyrimidinic endodeoxyribonuclease 1, anticorps APEX nuclease (multifunctional DNA repair enzyme) 1, anticorps APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog, anticorps DNA-(apurinic or apyrimidinic site) lyase, anticorps apurinic/apyrimidinic endonuclease 1, anticorps APEX1, anticorps apex1, anticorps apex1.L, anticorps CNG03640, anticorps CNH01280, anticorps nth, anticorps Spirs_4119, anticorps Palpr_0103, anticorps Apex1
- Sujet
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Name/Gene ID: APEX1
Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1 - ID gène
- 328
- UniProt
- P27695
- Pathways
- Réparation de l'ADN, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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