CD34 anticorps

N° du produit ABIN400780

Cet anticorps Rat Monoclonal détecte spécifiquement CD34 dans FACS, IP et IHC (p). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour CD34 anticorps (ABIN400780)

Antigène: CD34

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEC 14-7

Détail du produit anti-CD34 anticorps

Specificité: Recognizes the Mouse CD34 cell surface glycoprotein. Mouse CD34 is expressed by hematopoietic progenitor cells in the bone marrow and also by endothelial cells.

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Attributs du produit: Synonyms: Hematopoietic progenitor cell marker, Hematopoietic progenitor cell antigen CD34

Purification: Protein G Chromatography.

Isotype: IgG2a

Information d'application

Indications d'application: Immunoprecipitation: 2-10 μg/mL. Flow Cytometry: Use 10 μL of 1/25-1/50 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one monthor (Add 40-50% Sterile Glycerol) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker

ID gène: 12490

UniProt: Q64314