JAG1 anticorps (AA 110-125)

N° du produit ABIN401024

L’anticorps anti-JAG1 Polyclonal Lapin est utilisé pour la détection de JAG1 dans des échantillons de Humain, Souris, Rat et Chimpanzé. Il a été validé pour WB, IF, IHC (p) et EIA.

Aperçu rapide pour JAG1 anticorps (AA 110-125) (ABIN401024)

Antigène: JAG1 (Jagged 1 (JAG1))

Reactivité: Humain, Souris, Rat, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JAG1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-JAG1 anticorps

Épitope: AA 110-125

Specificité: This protein A purified antibody is directed against Human Jagged-1 protein. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in Mouse liver whole cell lysates, and a 75 kDa band in Human brain and kidney lysates. A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from Human, Chimpanzee, Rat and Mouse based on 100 % homology with the immunizing sequence. Partial reactivity is expected against dog (81 %) and Xenopus laevis (85 %) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee, Rat.
Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography on Protein A.

Immunogène: Synthetic peptide corresponding to amino acids 110-125 of Human Jagged-1 protein.

Isotype: IgG

Information d'application

Indications d'application: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence microscopy andWestern blot. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/500-1/2,000. Immunofluorescence: 1/200-1/1,000. Immunohistochemistry on Paraffin Sections: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur JAG1

Antigène: JAG1 (Jagged 1 (JAG1))

Autre désignation: CD339 / JAG1

Sujet: Jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the Jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.Synonyms: JAGL1, Jagged-1, hJ1

ID gène: 182

NCBI Accession: NP_000205

UniProt: P78504

Pathways: Signalisation Notch, Stem Cell Maintenance