CD97 anticorps (Extracellular Domain, N-Term)

N° du produit ABIN401030

L’anticorps anti-CD97 Polyclonal Lapin est utilisé pour la détection de CD97 dans des échantillons de Souris. Il a été validé pour WB, IF et EIA.

Aperçu rapide pour CD97 anticorps (Extracellular Domain, N-Term) (ABIN401030)

Antigène: CD97

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD97 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-CD97 anticorps

Épitope: AA 1-512, Extracellular Domain, N-Term

Specificité: This antibody is directed against the CD97 protein. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from Mouse (100 %) and Rat (74 %). Only 49 % homology is noted for the Human homologue. Reactivity against CD97 from other sources is not known.

Réactivité croisée (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse, Human, Chimpanzee.

Purification: Affinity chromatography on Protein A

Immunogène: Recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein

Isotype: IgG

Information d'application

Indications d'application: Immunofluorescence: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL deionized water (or equivalent).

Concentration: 5.0 mg/mL (UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur CD97

Antigène: CD97

Autre désignation: CD97

Sujet: CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including hematopoietic cells. Particularly high levels of expression are also seen in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T cells, B cells and NK cells. In humans, CD97 expression is upregulated during lymphocyte activation on various epithelial cancer cells including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha 5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Antibodies against CD97 are suitable for exploring various mouse genetic models of cancer, angiogenesis, and inflammation. CD97 can be proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Multiple isoforms have been identified for this protein.Synonyms: Leukocyte antigen CD97

ID gène: 26364

NCBI Accession: NP_001156501

UniProt: Q9Z0M6