PNKP anticorps (Internal Region)

N° du produit ABIN401253

Cet anticorps anti-PNKP Polyclonal Lapin (ABIN401253) détecte spécifiquement PNKP dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain et Chimpanzé.

Aperçu rapide pour PNKP anticorps (Internal Region) (ABIN401253)

Antigène: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Reactivité: Humain, Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PNKP est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-PNKP anticorps

Épitope: Internal Region

Specificité: This affinity purified antibody is directed against PNK protein.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee (100 %).Dog, mouse and rat (83 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide corresponding to an internal region of human PNK protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 300 - 1: 1,500 (Expect a band approximately 57 kDa in size corresponding toPNK in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.46 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Détails sur PNKP

Antigène: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Autre désignation: PNKP

Sujet: Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'- hosphatase and Polynucleotide kinase) catalyzes the 5' phosphorylation of nucleic acids and can have associated 3' phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.Synonyms: Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase

ID gène: 11284

NCBI Accession: NP_009185

UniProt: Q96T60

Pathways: Réparation de l'ADN, Nucleotide Phosphorylation