USP7 anticorps (N-Term)

N° du produit ABIN401255

L’anticorps anti-USP7 Polyclonal Lapin est utilisé pour la détection de USP7 dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Chimpanzé. Il a été validé pour WB et EIA.

Aperçu rapide pour USP7 anticorps (N-Term) (ABIN401255)

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp USP7 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-USP7 anticorps

Épitope: N-Term

Specificité: This affinity purified antibody is directed against HAUSP protein.

Réactivité croisée (Details): Species reactivity (expected):Rat, Mouse, Chimpanzee, Bovine and Dog (100 %).Chicken (92 %, 11/12).Zebrafish (83 %, 10/12).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to amino acids near the amino terminus of Human HAUSP protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/15,000-1/60,000. Western blot: 1/500-1/2,000 (Expect a band approximately 130 kDa in size correspondingto HAUSP protein in the appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.98 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or for longer (in aliquots) at-20 °C or below.

Détails sur USP7

Antigène: USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))

Autre désignation: USP7 / HAUSP

Sujet: HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53- dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically deubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.Synonyms: Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin carboxyl-terminal hydrolase 7, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

ID gène: 7874

NCBI Accession: NP_003461

UniProt: Q93009