RNF25 anticorps (C-Term)

N° du produit ABIN401373

Cet anticorps anti-RNF25 Polyclonal Lapin (ABIN401373) détecte spécifiquement RNF25 dans WB, IP et EIA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour RNF25 anticorps (C-Term) (ABIN401373)

Antigène: RNF25 (Ring Finger Protein 25 (RNF25))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNF25 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-RNF25 anticorps

Épitope: C-Term

Specificité: This antibody is directed against RNF25 protein.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur RNF25

Antigène: RNF25 (Ring Finger Protein 25 (RNF25))

Autre désignation: RNF25

Sujet: RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kappaB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25

ID gène: 64320

NCBI Accession: NP_071898

UniProt: Q96BH1