LacI anticorps

N° du produit ABIN401452

Cet anticorps Lapin Polyclonal détecte spécifiquement LacI dans WB et EIA. Il présente une réactivité avec des échantillons de E. coli et Shigella sonnei.

Aperçu rapide pour LacI anticorps (ABIN401452)

Antigène: LacI

Reactivité: E. coli, Shigella sonnei

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit

Specificité: This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100 % homology with the immunizing sequence.

Purification: Affinity Chromatography

Immunogène: Full-length recombinant Lac I protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/90,000-1/100,000. Western Blot: 1/500-1/2,000. Expect a band approximately approx. 38 kDa in size corresponding to Lac I by Western blotting inthe appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détail du antigène

Antigène: LacI

Sujet: The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.Synonyms: JW0336, Lactose operon repressor, b0345

ID gène: 945007

NCBI Accession: AP_000997

UniProt: P03023