MARCKS anticorps (pSer170)

N° du produit ABIN401558

L’anticorps anti-MARCKS Polyclonal Lapin est utilisé pour la détection de MARCKS dans des échantillons de Humain. Il a été validé pour WB et IHC (p).

Aperçu rapide pour MARCKS anticorps (pSer170) (ABIN401558)

Antigène: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MARCKS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MARCKS anticorps

Épitope: pSer170

Specificité: This antibody detects endogenous levels of MARCKS only when phosphorylated at Threonine 170.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 170 (G-F-Sp-F-K).

Information d'application

Indications d'application: Western blot: 1/500approx. 1/1000. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur MARCKS

Antigène: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Autre désignation: MARCKS

Sujet: MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL

ID gène: 4082

NCBI Accession: NP_002347

UniProt: P29966