Cyclin B1 anticorps (Ser147)

N° du produit ABIN401648

L’anticorps Lapin Polyclonal anti-Cyclin B1 a été validé pour WB, IF et IHC (p). Il convient pour détecter Cyclin B1 dans des échantillons de Humain et Souris.

Aperçu rapide pour Cyclin B1 anticorps (Ser147) (ABIN401648)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cyclin B1 anticorps

Épitope: Ser147

Specificité: Cyclin B1 Antibody detects endogenous levels of total Cyclin B1 protein.

Purification: Affinity chromatography

Immunogène: Synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V)

Information d'application

Indications d'application: Immunohistochemistry: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B1

Sujet: In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1

ID gène: 891

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase