Calnexin anticorps (Internal Region)

N° du produit ABIN401659

Cet anticorps Chèvre Polyclonal détecte spécifiquement Calnexin dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Porc. Il a été cité dans 2+ publications.

Aperçu rapide pour Calnexin anticorps (Internal Region) (ABIN401659)

Antigène: Calnexin (CANX)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Calnexin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Calnexin anticorps

Épitope: Internal Region

Séquence: c-SKTPELNLDQ FHDKT

Specificité: This antibody recognizes Calnexin.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Références pour anticorps anti-Calnexin (ABIN401659)

Ho, Chang, Su, Chang, Hu, Jou, Wang: "Inhibition of matrix metalloproteinase activity reverses corneal endothelial-mesenchymal transition." dans: The American journal of pathology, Vol. 185, Issue 8, pp. 2158-67, (2016) (PubMed).

Cordek, Croom-Perez, Hwang, Hargittai, Subba-Reddy, Han, Lodeiro, Ning, McCrory, Arnold, Koc, Lindenbach, Showalter, Cameron: "Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein." dans: The Journal of biological chemistry, Vol. 289, Issue 35, pp. 24397-416, (2015) (PubMed).

Détails sur Calnexin

Antigène: Calnexin (CANX)

Autre désignation: Calnexin

Sujet: Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90

ID gène: 821

NCBI Accession: NP_001737

UniProt: P27824

Pathways: Signalisation MAPK, Thyroid Hormone Synthesis