His Tag anticorps (Biotin)

N° du produit ABIN401733

Cet anticorps anti-His Tag Polyclonal Lapin conjugué à Biotin (ABIN401733) détecte spécifiquement His Tag dans WB, IHC (fro) et EIA.

Aperçu rapide pour His Tag anticorps (Biotin) (ABIN401733)

Antigène: His Tag

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-His Tag anticorps (Biotin)

Séquence: HHHHHH

Attributs du produit: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Purification: Affinity Chromatography

Immunogène: 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide

Information d'application

Indications d'application: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X Hisepitope. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for Immunoprecipitation andImmunocytochemistry. Recommended Dilutions:

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent). For extended storage, mix with Glycerol to 50 % final

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6xHistidine Epitope Tag

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged proteins biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.