L’anticorps anti-Albumin Polyclonal Lapin est utilisé pour la détection de Albumin dans des échantillons de Humain. Il a été validé pour WB, EIA et IHC (fro).
ALB
Reactivité: Macaque rhésus
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot: 1/1,000 - 1/5,000. ELISA: 1/170,000. Immunohistochemistry: 1/500 - 1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at -20 °C. After reconstitution, store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
Albumin (ALB)
Autre désignation
Albumin
Sujet
Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.Synonyms: ALB, BSA, HSA, Serum Albumin