CAMK2A
Reactivité: Humain
ELISA, IHC, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Peptide ELISA: 1/64000. Western blot: 0.1 - 0.3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Antigène
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
anticorps camk2, anticorps camk2b, anticorps zgc:112538, anticorps zgc:123320, anticorps CAMKA, anticorps CaMKII, anticorps R74975, anticorps mKIAA0968, anticorps PK2CDD, anticorps PKCCD, anticorps calcium/calmodulin dependent protein kinase II alpha, anticorps calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog, anticorps calcium/calmodulin-dependent protein kinase II alpha, anticorps CAMK2A, anticorps camk2a.S, anticorps camk2a, anticorps Camk2a
Sujet
Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1 % of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30 % of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968